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Suppressed natural killer cell activity in patients with chronic autoimmune thrombocytopenic purpura
Author(s) -
Semple John W.,
Bruce Suzanne,
Freedman John
Publication year - 1991
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830370409
Subject(s) - k562 cells , peripheral blood mononuclear cell , natural killer cell , cytolysis , cd8 , medicine , immunology , cd3 , cd16 , cytotoxicity , biology , immune system , leukemia , in vitro , biochemistry
Natural killer (NK) cell activity was studied in 17 patients with primary chronic idiopathic autoimmune thrombocytopenic purpura (ATP). Fifteen of 17 patients had a significantly reduced NK cytotoxicity against 51 chromium labeled K562 target cells (mean LU 20% = 18 ± 20 in patients versus 65 = 2 5 in controls, P < 0.001). NK activity was also significantly reduced in all of six patients with secondary ATP as compared with normal controls (LU 20% 28±15, respectively, P < 0.005). The NK activity in both patient groups correlated with the duration of therapy being received (r = 0.60, P < 0.001). Immunophenotypic analysis of peripheral blood mononuclear cells from patients with ATP revealed that CD8‐ cells bearing CD57 (HNK‐1, Leu 7) and CD3‐ cells bearing CD56 (Leu 19) were quantitatively within the normal range. These findings indicate that patients with ATP have a functional defect in NK cytolytic activity.