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CD5 positive immunoregulatory B cells in spleen populations from multiple myeloma patients
Author(s) -
MacKenzie Malcolm R.,
Paglieroni Teresa,
Caggiano Vincent
Publication year - 1991
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830370306
Subject(s) - cd5 , spleen , cd19 , multiple myeloma , b cell , pokeweed mitogen , myeloma protein , immunology , antibody , biology , b 1 cell , lymphatic system , t cell , immune system , antigen presenting cell , in vitro , peripheral blood mononuclear cell , biochemistry
CD19+CD5+ lymphocytes constitute a minority of peripheral blood B cells. In view of the importance of these cells in the pathogenesis of the immunoregulation of myeloma, their incidence in another lymphoid organ was determined. CD5+ B cells were studied in 9 spleens from patients with multiple myeloma and in 10 spleens from normal individuals removed secondary to trauma. The total number of CD19+ B cells were increased in myeloma spleens (44.4% ± 12.6%) as compared to normal spleens (20.4% ± 7.4%). Likewise, the percentage of CD19 cells which co‐expressed CD5 were increased in myeloma (25.3% ± 12.4%) versus normal (4.4% ± 2.3%) spleen. CD5+ B cells isolated from myeloma spleens, but not normal spleens, inhibit production of immunoglobulin in a pokeweed mitogen driven assay. Thus the spleen appears to be an important source of immunoregulatory B cells in multiple myeloma.