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Evidence for the synthesis of embryonic globin chains in adult erythroid progenitor cells
Author(s) -
Bhaumik Kabita
Publication year - 1991
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830360105
Subject(s) - embryonic stem cell , globin , progenitor cell , progenitor , microbiology and biotechnology , biology , genetics , stem cell , gene
Embryonic globin chains were found to be synthesized in vitro by the BFU‐E colonies derived from adult sickle cell anemia (SS) patients, their heterozygous relatives as well as a few normal controls. In the absence of sufficient material for conducting direct structural analyses of these peptides, they were confirmed by evaluating the co‐migration of the ϵ‐ and ζ‐chains with the corresponding structurally characterized globin chains obtained from K562 cell lysates on a reversed phase high performance liquid chromatogram. The presence of ζ‐chain was also confirmed using an immunologic procedure. Furthermore, significant 35 S‐methionine incorporation peak was observed corresponding to the ζ‐chain synthesized by the BFU‐E‐derived colonies implying an active synthesis of this embryonic globin chain in BFU‐E cells obtained from hemopoietically adult persons.