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FAB L3 Type of B‐cell acute lymphoblastic leukemia (B‐ALL) without chromosome abnormalities
Author(s) -
Imamura Nobutaka,
Mtasiwa Deodatus M.,
Ota Hiromi,
Inada Tominari,
Kuramoto Atsushi
Publication year - 1990
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830350316
Subject(s) - cd19 , biology , karyotype , immunophenotyping , microbiology and biotechnology , cd3 , cd20 , cd8 , monoclonal antibody , antigen , myeloid , chromosomal translocation , cytogenetics , antibody , immunology , chromosome , genetics , gene
Acute lymphocytic leukemias (ALLs) are morphologically classified into L1, L2, and L3. The former two types are phenotypically constituted of quite heterogeneous ALLs. In the present study, phenotypes of cells from five L3 type ALL were analysed in FACS‐IV using a panel of monoclonal antibodies (MAbs). The leukemic cells of all these patients coexpressed la (HLA‐DR), CD19, CD20, CD21, CD24, CD38, and surface immunoglobulins, whereas a negative reaction with MAbs CD1, CD2, CD3, CD4, CD7, CD8, and Ti (WT31), TiγA, δTCS 1, and anti TCR‐γ/δ was observed. Neither myeloid‐monocyte‐erythroid nor megakaryocyte related cell surface antigens were detected in these cases with L3 type ALL. Chromosomal analysis of the ALL cells from two cases revealed a normoploid karyotype with specific translocation t(8;14)(q24;q32), whereas it was normal (46XY or 46XX) for the remaining three cases. Expression of myc oncogene was high in the former group, but low in the later one. Basing from our findings, we conclude that L3 type ALL is heterogeneous with respect to immunophenotypes, cytogenetics and oncogene analysis.