Premium
Distribution of cell surface glycoprotein CD9 (P24) antigen on megakaryocyte lineage leukemias and cell lines
Author(s) -
Imamura Nobutaka,
Mtasiwa Deodatus M.,
Ota Hiromi,
Inada Tominari,
Kuramoto Atsushi
Publication year - 1990
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830350115
Subject(s) - megakaryocyte , glycoprotein , lineage (genetic) , antigen , cell , distribution (mathematics) , immunology , cancer research , biology , microbiology and biotechnology , haematopoiesis , gene , genetics , stem cell , mathematical analysis , mathematics
We herein describe CD9 (p24) antigen as existing on the cell surface of megakaryocyte lineage leukemias as well as megakaryocytic leukemia cell lines, MEG‐01 and HEL, by means of fluorescence‐activated cell sorter (FACS IV) with a panel of monoclonal antibodies (Mabs). We found CD9 antigen expression on the cell surface of megakaryoblastic leukemias as well as MEG‐01 and HEL cells. Furthermore, CD9 antigen expression increased while culturing these cells with phorbol esters, and was also found in the cytoplasm by means of indirect immunofluorescence test. These findings clearly showed that CD9 antigen exists on the cell surface of megakaryocytic cells which are capable of synthesizing the CD9.