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Type‐C virus‐like particles in a human B‐cell lymphoma cell line
Author(s) -
Kubonishi Ichiro,
Sawada Takashi,
Yano Shoki,
Eguchi Taisuke,
Iwahara Yoshihito,
Takeuchi Chise,
Miyoshi Isao,
Ohtsuki Yuji
Publication year - 1990
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830350114
Subject(s) - lymphoma , virology , virus , cell culture , antigen , biology , chronic lymphocytic leukemia , b cell , leukemia , microbiology and biotechnology , antibody , immunology , genetics
Type‐C virus‐like particles (VLPs) were found in an Epstein‐Barr (EB) virus‐infected human B‐cell lymphoma cell line, SP‐50B, that was established from a patient with non‐Hodgkin lymphoma. The cell line continuously produces a small number of type‐C VLPs, 150–200 nm in diameter, over 1 year. SP‐50B cells were negative for HTLV‐I and HTLV‐II antigens and did not contain the HTLV‐I genome. In addition, two EB virus nuclear antigen (EBNA)‐positive B‐cell lines, SP‐54‐Cord and SP‐57‐CLL, were established from human cord blood and chronic lymphocytic leukemia (CLL), respectively, by coculture with lethally irradiated SP‐50B cells. Type‐C VLPs with the same morphology were also found in both cell lines.