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Bone marrow in vitro growth and cytogenetic studies in patients with fab‐classified primary myelodysplastic syndromes
Author(s) -
Swolin Birgitta,
Rödjer Stig,
Westin Jan
Publication year - 1990
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830340304
Subject(s) - myelodysplastic syndromes , bone marrow , cfu gm , karyotype , biology , cytogenetics , leukemia , chromosome abnormality , acute leukemia , pathology , immunology , chromosome , medicine , haematopoiesis , genetics , stem cell , gene
Thirty‐eight consecutive patients with a FAB‐classified primary myelodysplastic syndrome (MDS) were investigated for in vitro growth of colony‐forming units for granulocyte‐macrophage precursors (CFU‐GM) and cytogenetic analysis of bone marrow cells. Abnormal CFU‐GM growth was found in 30 patients (79%), and clonal chromosome abnormalities were found in 13 patients (34%). The eight patients who showed normal CFU‐GM growth were either cytogenetically normal (n = 5), or had a 5q‐ deletion (n = 3) as single or dominating karyotypic abnormality. Among the 30 patients with reduced or no colony growth, ten patients had a clonal chromosome abnormality. Leukemia developed in eight patients. None of them grew any CFU‐GM colonies, and three of them were cytogenetically abnormal at the time of diagnosis of MDS. Analysis of the bone marrow in vitro growth for CFU‐GM and the karyotype in patients with MDS emphasizes the close relationship between these disorders and manifest acute leukemia. Subgroups of MDS may be defined by a cytogenetic classification (e.g., the 5q‐ syndrome), and the CFU‐GM growth pattern can be of value for predicting leukemic transformation.