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Varied expressions of receptors on the human megakaryoblastic cell line MEG‐01 by monoclonal antibodies
Author(s) -
Imamura Nobutaka,
Mtasiwa Deodatus M.,
Inada Tominari,
Kuramoto Atsushi,
Ogura Michinori,
Saito Hidehiko
Publication year - 1990
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830340214
Subject(s) - monoclonal antibody , receptor , biology , microbiology and biotechnology , cell culture , antibody , chemistry , immunology , biochemistry , genetics
Abstract We herein describe the regulation of specific receptors on the megakaryoblastic cell line MEG‐01 by means of fluorescence‐activated cell sorting (FACS IV) with a panel of monoclonal antibodies (MAbs). MEG‐01 cells expressed GpIIb/IIIa (CD41a) and OKM5 (CD36) antigens on their cell surface, whereas they showed only a little expression of GPIb (CD42b), suggesting that these are megakaryoblastic cells. The up regulation of von Willebrand factor receptor (GPIb) and thrombospondin receptor (GPIV) and the down regulation of fibrinogen receptor (GPIIb/IIIa) and C3bi receptor (CD11b) were found by incubation with MAbs AN51 (CD42b), OKM5 (CD36), J15 (CD41a), and OKM1 (CD11b), respectively. This phenomenon was enhanced in the Ca 2+ containing medium, except for the experiment with OKM5 (CD36) MAb. We thus suggest that several kinds of receptors on the surface of MEG‐01 cells are dexterously regulated through stimulation from outside the cell.