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Rapid reduction of methemoglobin in rat bone marrow erythroid cells
Author(s) -
Yubisui Toshitsugu,
Takeshita Masazumi,
Hultquist Donald E.
Publication year - 1990
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830340103
Subject(s) - methemoglobin , bone marrow , incubation , chemistry , nitrite , biochemistry , hemoglobin , endocrinology , medicine , immunology , biology , organic chemistry , nitrate
Methemoglobin reduction was shown to proceed much more rapidly in erythroid cells from rat bone marrow than in rat erythrocytes. Methemoglobin reduction in suspensions of intact, nitrite‐treated bone marrow cells does not depend on the presence of glucose in the incubation mixture, even after the cells have been stored in substrate‐free medium. 2‐Deoxyglucose and iodoacetate prevent the reduction from proceeding to completion. The results suggest that, relative to erythrocytes, immature erythroid cells more efficiently catalyze methemoglobin reduction and more effectively store metabolites which provide electrons for this reaction.