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Detection of engraftment and chimerism after bone marrow transplantation by in situ hybridization using a Y‐chromosome specific probe
Author(s) -
Agematsu Kazunaga,
Kitahara Fuminori,
Uehara Yoshio,
Kawai Hiroshi,
Miyagawa Yukiaki,
Komiyama Atsushi,
Nakahori Yutaka,
Nakagome Yasuo,
Akabane Taro
Publication year - 1990
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830330408
Subject(s) - in situ hybridization , bone marrow , southern blot , bone marrow transplantation , hybridization probe , biology , haematopoiesis , hematopoietic cell , pathology , transplantation , dot blot , in situ , microbiology and biotechnology , immunology , medicine , dna , stem cell , genetics , chemistry , gene , gene expression , organic chemistry
Abstract After bone marrow transplantation (BMT), the recipient and donor cells must be distinguished from each other to document and characterize successful engraftment. In addition to dot blot and Southern blot analyses, we have performed in situ hybridization in two sex‐mismatched cases using a Y‐chormosome specific DNA probe (PHY10). In situ hybridization showed that >95% of the peripheral mononculear cells had clusters of grains indicative of male cell origin in a recipient girl (case 1), and no cells had clusters of grains in another recipient boy (case 2) at the time of engraftment and 3 months after BMT. In situ hybridization using the PHY10 probe appears to facilitate identification of individual cells of male and female origin, and it requires only 20 hr to obtain the results. The technique provides a powerful new method for the documentation of engraftment and the detection of mixed hematopoietic chimerism in peripheral blood and bone marrow cell compartments after BMT.