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Effect of histocompatibility factors on pulmonary retention of indium‐111‐labeled granulocytes
Author(s) -
Dutcher Janice P.,
Riggs Charles,
Fox Jeremiah J.,
Johnston Gerald S.,
Norris Dottie,
Wiernik Peter H.,
Schiffer Charles A.
Publication year - 1990
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830330405
Subject(s) - granulocyte , medicine , antibody , histocompatibility , immunology , lung , human leukocyte antigen , antigen
Granulocyte transfusions are associated with a number of side effects including febrile transfusion reactions and occasionally pulmonary infiltrates. There is evidence that the presence of preformed antibodies may be a cause of these complications. In this study, allogeneic 111 lndium‐labeled granulocytes were used to evaluate the pulmonary retention of radioactivity in alloimmunized and non‐alloimmunized patients in an attempt to assess antibody effect on granulocyte migration. After injection of labeled allogeneic granulocytes into neutropenic patients, the ratios of lung to heart activity were calculated for the first 30 min of scanning. There was significantly greater retention of radioactivity from cells in the lungs of patients who were alloimmunized, having both lymphocytotoxic (anti‐HLA) and leukoagglutinating antibodies, compared to the activity in the lungs of non‐alloimmunized patients ( P < 0.001) or of patients receiving autologous granulocytes ( P < 0.001). This study demonstrates that labeled, mismatched granulocytes may be retained in the lungs for a significantly longer time in patients with preformed antibodies. This implies that transfusion of large numbers of such mismatched granulocytes, i. e., granulocyte transfusions, may also be retained in the lungs of alloimmunized patients, which could lead to pulmonary compromise. Therefore, granulocyte transfusions from random donors should not be given to alloimmunized patients.