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B‐cell surface phenotypes of proliferating myeloma cells: Target antigens for immunotherapy
Author(s) -
Chan Clara S. P.,
Wormsley Susan B.,
Pierce Lawrence E.,
Peter James B.,
Schechter Geraldine P.
Publication year - 1990
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830330206
Subject(s) - antigen , cd19 , biology , clone (java method) , antibody , b cell , multiple myeloma , immunology , cd38 , cancer research , microbiology and biotechnology , stem cell , cd34 , gene , biochemistry
Dual‐parameter flow cytometric analysis of B‐cell antigens and DNA content was used to determine the phenotypes of proliferating tumor cells (S‐phase cells) from 30 patients with multiple myeloma. B4 (CD19), J5 (CALLA, CD10), B1 (CD20), and monotypic surface immunoglobulin (Sig) were expressed heterogeneously in 24 patients. J5 and monotypic Slg were found most frequently but were always expressed on a significantly lower percentage of cells than the antigens typically associated with plasma cells, cytoplasmic immunoglobulin (Clg) and T10 (CD38). S‐phase cells were found in each antigen(+) subset. B antigen(+) cycling cells were demonstrated in 16 patients whose marrow or blood cells expressed B antigens exclusively in the hyperdiploid fraction and therefore were certainly part of the myeloma clone. Similar to the low level of proliferative activity of the T10(+), Clg(+), and PCA1(+) subsets, the percentages of cycling cells of the preplasma cell B‐antigen‐bearing myeloma subsets ranged from < 1% to 12%. The tumor cells of four patients were also studied with dual‐color surface antigen analysis and demonstrated independent expression of B antigens, with only rare coexpression of T10 and monotypic Slg, J5, or B4. These findings are consistent with the presence of distinct myeloma subsets bearing differing B phenotypes in the same tumor and provide evidence that the proliferation in myeloma is occurring at various developmental stages in the malignant B lineage. These antigens may be important targets for immunologic therapy aimed at eliminating the entire proliferating compartment of this B‐cell tumor.