Artefactual low lymphocyte activity caused by platelet contamination in the mononuclear cell preparations | Zendy

Rueda F. | Zendy; Marti F. | Zendy; Pinol G. | Zendy; Remacha A. | Zendy

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Artefactual low lymphocyte activity caused by platelet contamination in the mononuclear cell preparations

Author(s) -

Rueda F.,

Marti F.,

Pinol G.,

Remacha A.

Publication year - 1989

Publication title -

american journal of hematology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.456

H-Index - 105

eISSN - 1096-8652

pISSN - 0361-8609

DOI - 10.1002/ajh.2830310210

Subject(s) - lymphoproliferative response , peripheral blood mononuclear cell , platelet , lymphoproliferative disorders , lymphocyte , immunology , medicine , contamination , interleukin 2 , chemistry , in vitro , biology , cytokine , lymphoma , biochemistry , ecology

Platelet (PLT) contamination in preparations of mononuclear cells (MNC) produces a significant decrease ( P <0.02) in lymphoproliferative activity and interleukin 2 (IL2) production. This effect was observed in MNC preparations from either healthy blood donors or polyglobulic patients. In a dose‐response curve carried out with increasing PLT number per MNC, progressive inhibition was found, reaching to 62.6% and 49.5%, respectively, to phytohemagglutinin and IL2 lymphoproliferative response and 87.8% in IL2 production, at 80 PLTs/MNC.

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