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Artefactual low lymphocyte activity caused by platelet contamination in the mononuclear cell preparations
Author(s) -
Rueda F.,
Marti F.,
Pinol G.,
Remacha A.
Publication year - 1989
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830310210
Subject(s) - lymphoproliferative response , peripheral blood mononuclear cell , platelet , lymphoproliferative disorders , lymphocyte , immunology , medicine , contamination , interleukin 2 , chemistry , in vitro , biology , cytokine , lymphoma , biochemistry , ecology
Platelet (PLT) contamination in preparations of mononuclear cells (MNC) produces a significant decrease ( P <0.02) in lymphoproliferative activity and interleukin 2 (IL2) production. This effect was observed in MNC preparations from either healthy blood donors or polyglobulic patients. In a dose‐response curve carried out with increasing PLT number per MNC, progressive inhibition was found, reaching to 62.6% and 49.5%, respectively, to phytohemagglutinin and IL2 lymphoproliferative response and 87.8% in IL2 production, at 80 PLTs/MNC.