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Genotypic analysis using a Y‐chromosome‐specific probe following bone marrow transplantation
Author(s) -
Morisaki Hiroko,
Morisaki Takayuki,
Nakahori Yutaka,
Ogura Hiromi,
Kanno Hitoshi,
Tani Kenzaburo,
Kodo Hideki,
Fujii Hisaichi,
Asano Shigetaka,
Miwa Shiro
Publication year - 1988
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830270108
Subject(s) - bone marrow , bone marrow transplantation , chromosome , southern blot , transplantation , chromosome analysis , biology , microbiology and biotechnology , y chromosome , pathology , dna , cancer research , immunology , medicine , genetics , karyotype , gene
To monitor successful engraftment after bone marrow transplantation, we performed Southern hybridization analysis or dot blot analysis of DNA in a set of sex‐mismatched cases using a Y‐chromosome‐specific DNA probe (pHY10). This method was extremely sensitive and rapid for checking which cells contain the Y‐chromosome. Using this probe, analysis of cells from peripheral blood and bone marrow after transplantation demonstrated the usefulness of confirming engraftment of donor cells and of detecting mixed lymphohematopoietic chimerism.