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Relationship of fluorescence polarization to cell lineage in lymphocytes from normal subjects and patients with chronic lymphocytic leukemia
Author(s) -
Glotzer Taya V.,
Liebes Leonard F.,
Kanganis Denise,
Silber Robert
Publication year - 1987
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830260304
Subject(s) - chronic lymphocytic leukemia , leukemia , fluorescence anisotropy , significant difference , medicine , immunology , pathology , chemistry , biochemistry , membrane
Previous investigations have shown differences in fluorescence polarization between normal and chronic lymphocytic leukemia lymphocytes following incubation with the probe 1,6‐diphenyl‐1,3,5‐hexatriene. In the present study, we determined the fluorescence polarization of unseparated or enriched subpopulations of T and B lymphocytes from normal subjects and patients with chronic lymphocytic leukemia. As had been observed by others, the mean polarization (P) value at 25°C for unseparated chronic lymphocytic leukemia lymphocytes, .240 ± .007 (N = 22), was lower than that of unseparated normal lymphocytes, .248 ± .005 (N = 18), P < .001 (Student's t‐test). The difference was greater when B‐enriched populations were compared. The mean P value of B‐cell‐enriched chronic lymphocytic leukemia lymphocytes, .240 ± .007 (N = 5), was significantly lower than that of B‐cell‐enriched normal preparations, .256 ± .004 (N = 5), P < .001. In contrast, no significant difference was found between normal and chronic lymphocytic leukemia T cells. The anomalous fluorescence polarization manifested by chronic lymphocytic leukemia lymphocytes of B‐cell origin serves to distinguish this lineage from its normal counterpart and from T cells of either source.