Premium
Hairy leukemic cells which hyperexpress I i do not demonstrate I i genome alterations by restriction endonuclease analysis
Author(s) -
Owerbach David I.,
Elliott William L.,
Humphreys Robert E.
Publication year - 1987
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830250308
Subject(s) - hairy cell leukemia , biology , restriction enzyme , microbiology and biotechnology , genomic dna , genome , complementary dna , dna , hairy cell , endonuclease , spleen , leukemia , point mutation , gene , genetics , mutation , immunology
Abstract The finding of increased expression and apparently altered processing of I i in hairy leukemic cells led us to test for I i genomic alteration by Southern‐type I i cDNA hybridization to leukemic spleen DNA cleaved with a series of restriction endonucleases. Some insertions, deletions, or point mutations, potentially detectable by this technique, might correlate to alteration in expression and function of I j (and indirectly, class II antigens). No changes in genomic structure of I i were detected in DNA isolated from spleens of five patients with hairy cell leukemia, compared with DNA preparations from peripheral blood cells of nineteen healthy blood donors. These experiments were consistent with the view that gross structural alteration of the I i genome had not occurred in hairy leukemic cells.