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Serum from patients with non‐hodgkin's lymphoma(nhl) inhibits T‐cell colony formation
Author(s) -
Hutchison Robert E.,
Tice Diane G.,
Davey Frederick R.
Publication year - 1987
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830250204
Subject(s) - peripheral blood mononuclear cell , lymphoma , lymph node , null cell , lymph , immunology , antigen , t cell , cell , medicine , pathology , biology , cell culture , immune system , in vitro , biochemistry , genetics
This study compared T‐cell colony formation in soft agar of lymphocytes from the peripheral blood and lymph nodes of patients with non‐Hodgkin's lymphoma (NHL) with T‐cell colony formation of peripheral blood lymphocytes from normal individuals. Mononuclear cells were separated from other blood and lymph node elements on density gradient columns, phenotyped for T‐ and B‐cell antigens using monoclonal antibodies, and then plated in soft agar cultures. Lymphocytes from peripheral blood and lymph nodes of patients with NHL exhibited less T‐cell colony formation (p < 0.01) than did lymphocytes from normal individuals. This decrease in T‐cell colony formation was not the result of the number of T cells or null cells plated, or differences in proportions of T helper and T suppressor cells. However, when sera from patients with NHL were incubated with normal lymphocytes before plating in soft agar, a decrease in number of T‐cell colonies was observed (p < 0.01). We conclude that peripheral blood and lymph node mononuclear cells from patients with NHL have a decreased ability to form T‐cell colonies in soft agar cultures and that this decrease is related, at least in part, to the presence of serum factor(s).