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Inhibitory effects of TTP sera on binding of anti‐platelet glycoprotein II b ‐III a monoclonal antibodies to human vascular endothelial cells
Author(s) -
Nakajima Tokuo,
Koyama Tetsuji,
Kakishita Eizo,
Nagai Kiyoyasu
Publication year - 1987
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830250113
Subject(s) - monoclonal antibody , platelet , umbilical vein , glycoprotein , antibody , microbiology and biotechnology , monoclonal , chemistry , endothelial stem cell , platelet membrane glycoprotein , immunology , medicine , biochemistry , biology , in vitro
The binding of anti‐human platelet glycoprotein (GP) II b III a monoclonal antibodies to human umbilical vein endothelial cells (HUVE) was studied. Scatchard analysis using 125 I‐anti‐platelet GPII b ‐III a monoclonal antibody showed that the maximum binding capacity (B max) was 8 × 10 4 /cell and Kd was 40.2 nM. The binding rate of 125 I‐anti‐platelet GPII b III a monoclonal antibodies to HUVE treated with TTP sera was 11.6 ± 2.8%, compared with the 24.0 ± 7.5% observed for HUVE treated with normal sera. These findings showed that there are GPII b ‐III a on HUVE and that TTP sera may contain anti‐platelet GPII b III a antibodies.