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Phenotypic changes associated with chemically induced differentiation of a hairy cell leukemia cell line
Author(s) -
Hooper W. Craig,
Barth Rolf F.,
Minowada Jun
Publication year - 1987
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830240410
Subject(s) - calla , cell culture , hairy cell leukemia , phenotype , receptor , cellular differentiation , leukemia , biology , hairy cell , in vitro , monocyte , lymphoblast , thp1 cell line , precursor cell , microbiology and biotechnology , immunology , biochemistry , antibody , genetics , monoclonal antibody , gene
Abstract In vitro chemically induced differentiation of the JOK‐1 cell line, derived from a patient with hairy cell leukemia, has been studied. Non‐induced JOK‐1 cells expressed sIgM, B‐1, CALLA, Ia, and C3 receptors. Following exposure to 2% DMSO, or 100 ng/ml 12‐0‐tetradecanoyl‐phorbol‐13‐acetate (TPA), the cells underwent a phenotypic change from lymphoblastoid to monocytoid. In the presence of TPA, they became adherent and developed dendritic processes. Associated with these changes was a decrease in sIgM and CALLA and variable expression of C3 receptors. No monocyte/macrophage‐associated antigens were detected. These results suggest that the JOK‐1 cell line may have a limited ability to differentiate but is not mature enough to complete the differentiation process.