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Isolation of malignant cells from human bone marrow using a discontinuous percoll gradient
Author(s) -
Macfarlane S. D.,
Tauro G. P.,
Rendall K.,
Welch J. J.,
Ekert H.
Publication year - 1986
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830220409
Subject(s) - percoll , malignancy , bone marrow , haematopoiesis , hematological malignancy , pathology , medicine , malignant cells , hematologic malignancy , malignant disease , stem cell , biology , cancer , centrifugation , biochemistry , genetics
A technique for examining relatively large volumes of bone marrow for involvement by malignancy is described. The use of discontinuous Percoll gradients offers no advantage over conventional methods in the diagnosis of hematological malignancy. Its usefulness in detecting infiltration by solid tumor is uncertain. Complete exclusion of malignancy from the fraction containing hematologic stem cells in three patients raises the possibility that this technique is a useful adjunct to other methods of marrow purging before autologous marrow rescue in malignant disease.