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Autologous survival of cyanate‐treated cryopreserved sickle erythrocytes
Author(s) -
Castro Oswaldo,
Rana Sohall R.,
Poillon William N.
Publication year - 1986
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830210409
Subject(s) - cyanate , cryopreservation , andrology , chemistry , in vitro , biochemistry , medicine , biology , embryo , polymer chemistry , microbiology and biotechnology
The effects of carbamylation and frozen storage on the autologous 51 Cr survival and metabolic features of sickle erythrocytes (S‐RBCs) were determined. Red cells from four patients with sickle hemoglobinopathies were treated with 50 mM sodium cyanate for 2 hr (37°C), glycerolized and frozen (−30°C) for 62‐153 days. The mean in vitro loss of S‐RBCs from the combination of cyanate treatment and cryopreservation was 23.6% (±3.5 SD). The 2, 3‐diphosphoglycerate content of the thawed cells did not change significantly. However, ATP levels decreased to about 50% of the corresponding values in fresh, untreated S‐RBCs. Despite this decrease in ATP, the mean intravascular survival of the frozen cyanated cells nearly doubled. At the high concentration of cyanate used, the oxygen affinity of S‐RBCs increased markedly: Their mean P50 was 13.1 mm Hg (± 1.9SD). The gelation of HbS at zero pO 2 was also markedly inhibited in the one sample of cyanate‐treated S‐RBCs examined. Clinical studies to determine the efficacy of autologous transfusions with extensively carbamylated, cryopreserved S‐RBCs should be considered.