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Deoxythymidine and deoxycytidine incorporation into DNA in B leukemic cells
Author(s) -
Vertongen F.,
Lebacq A.M.,
Fondu P.,
Mandelbaum I. M.
Publication year - 1986
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830210312
Subject(s) - deoxycytidine , dna , deoxycytidine kinase , microbiology and biotechnology , incubation , peripheral blood mononuclear cell , dna synthesis , biology , chemistry , cancer research , biochemistry , in vitro , chemotherapy , genetics , gemcitabine
Measurements of radioactive DNA after incubation of normal and B leukemic peripheral mononuclear cells, from c‐ALL and CLL with labeled deoxythymidine (dTh) and deoxycytidine (dCt) showed that for dTh, incorporation into DNA was similar for normal and c‐ALL cells but lower in B‐CLL cells and that for dCt, incorporation was highest in c‐ALL and lowest in CLL cells. These results contrast with those of dTh and dCt kinase activities; the former has been previously found elevated in c‐ALL cells, and the latter is found, in the present study, similar in the three groups tested.