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The laboratory use of butylnitrite for the production of methemoglobin
Author(s) -
Roth Eugene,
Rinaldi Antoniettina,
Fiori Giovanni Maria,
Filippi Giorgio
Publication year - 1985
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830200208
Subject(s) - methemoglobin , sodium nitrite , hemoglobin , nitrite , in vitro , intracellular , chemistry , biochemistry , heterozygote advantage , methemoglobinemia , medicine , andrology , food science , genotype , organic chemistry , nitrate , gene
The use of volatile butylnitrite in place of sodium nitrite for the in vitro production of methemoglobin was explored in studies of G6PD‐deficient red cells and for measurements of the red cell methemoglobin reductase activity. It was found that butylnitrite vapor caused a more rapid oxidation of intracellular hemoglobin than sodium nitrite and required fewer washes for removal. Hence a more rapid preparation of the cells was possible. Both cytochemical detection of G6PD‐deficient cells in a female heterozygote for G6PD deficiency and the screening test for a methemoglobin reductase deficiency could be performed with butylnitrite as well as with sodium nitrite. This small modification of these standard procedures promises to save time and facilitate processing of many samples during genetic screening of relevant populations.