Premium
Low molecular weight iron in guinea pig reticulocytes
Author(s) -
Pollack Simeon,
Campana Theresa,
Weaver Janet
Publication year - 1985
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830190110
Subject(s) - reticulocyte , ferrous , orcinol , chemistry , elution , guinea pig , biochemistry , nucleotide , chromatography , amino acid , thin layer chromatography , biology , rna , organic chemistry , endocrinology , gene
The low molecular weight iron found in the guinea pig reticulocyte has been partially characterized. On thin layer chromatography it is distinguishable from the iron complexes of a variety of nucleotides, sugars, and amino acids. On paper chromatography it comigrates with a 250‐nm absorbing, orcinol‐positive material. The eluted count peak contains phosphorus. Approximately 1 μg of iron is recovered from 1 ml of hemolyzed red cells. Preparation under nitrogen improves recovery of low molecular weight iron, suggesting that the iron is in the ferrous oxidation state.