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A microtiter method for long‐term in vitro maintenance of human monocyte
Author(s) -
Kataoka Mikio,
Tavassoli Mehdi
Publication year - 1985
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830180313
Subject(s) - microtiter plate , in vitro , monocyte , human blood , term (time) , yield (engineering) , chemistry , immunology , biology , chromatography , biochemistry , physiology , materials science , metallurgy , physics , quantum mechanics
A microtiter method is described for long‐term maintenance of human monocytes in culture with intact functional and morphological characteristics. This micromethod is the adaptation of our previously reported method which required large volume of blood. The micromethod relies on the use of microexudate plate (pregrown with BKH cells) and the two phases of adherence and maintenance culture. The purity of cultured cells approaches 100% and the yield is 50–70%. As many as 24 experiments and controls can be done on 20 ml of blood drawn.