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Hemoglobin miyada: DNA analysis of the anti‐lepore βδ fusion gene
Author(s) -
Driscoll M. Catherine,
Ohta Yoshiro,
Nakamura Frank,
Bloom Arthur,
Bank Arthur
Publication year - 1984
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830170405
Subject(s) - gene , biology , microbiology and biotechnology , genetics , coding region , restriction enzyme , dna , globin , mutant
Hemoglobin Miyada, an anti‐Lepore hemoglobin, represents the protein product of a nonhomologous crossover between β and δ genes. The mutant globin is β‐like from the N terminus to amino acid 12, and δ‐like from amino acid 22 through the C terminus, thus predicting a crossover site in the first coding region of the gene. DNA analysis, using multiple restriction endonucleases and hybridization to δ and β globin gene‐specific probes, confirms that the βδ hybrid gene 1) is located on a single chromosomal fragment between normal δ and β genes, and 2) has 5′ β promoter sequences, δ IVS 1 and 2, and 3′ coding and flanking sequences.