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Functional diversity within the suppressor phenotype as defined by monoclonal antibody in T‐cell prolymphocytic leukemia
Author(s) -
Zamkoff Kenneth W.,
Poiesz Bernard J.,
Ruscetti Francis W.,
Moore Janet L.,
Davey Frederick R.,
Planas Antonio T.,
Lamberson Harold
Publication year - 1984
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830160412
Subject(s) - prolymphocytic leukemia , phenotype , monoclonal antibody , pokeweed mitogen , leukemia , immunology , biology , cancer research , suppressor , monoclonal , antibody , in vitro , cancer , chronic lymphocytic leukemia , genetics , gene , peripheral blood mononuclear cell
A patient with T‐cell prolymphocytic leukemia (T‐PLL) is described. The malignant T‐cells from the patient were predominantly Leu‐2‐positive, indicating a suppressor phenotype. The cells were then tested to determine their functional capabilities. The patient's Leu‐2‐positive cells initially suppressed B‐cell proliferation, as predicted by their phenotype but later functioned as T helper cells in the pokeweed mitogen system without a change in phenotype. The cells also responded inadequately to alloantigen and mitogen despite addition of exogenous T‐cell growth factor (TCGF). Leu‐2‐positive prolymphocytes from the spleen of the patient were constitutive producers of TCGF. Surface phenotype using monoclonal antibody was inadequate to predict T‐cell function of the cells from this patient with T‐PLL. In addition, these data suggest there may be functional subpopulations within the OKT8 + phenotype. Constitutive TCGF production by malignant post‐thymic T‐cells may represent a mechanism by which these cells sustain their own growth.