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Sideroblastic anemia: Differences in bone marrow erythroid colony (CFU e ) growth responses to erythropoietin in plasma clot and methylcellulose cultures
Author(s) -
Lutton J. D.,
Ibraham N. G.,
Hoffman R.,
Ritchey A. K.,
Levere R. D.
Publication year - 1984
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830160303
Subject(s) - erythropoietin , bone marrow , sideroblastic anemia , anemia , in vitro , biology , immunology , erythropoiesis , microbiology and biotechnology , endocrinology , medicine , biochemistry
Bone marrow cells from three patients with idiopathic acquired sideroblastic anemia and one with X‐linked sideroblastic anemia were simultaneously cultured in plasma clot and methylcellulose cultures in order to evaluate their erythroid colony (CFU E ) cloning potential in these two systems. In contrast to normals, sideroblastic anemia bone marrow cells demonstrated a marked ability to form erythropoietin (Epo)‐independent CFU E in methylcellulose culture, and were inhibited in their ability to generate CFU E in plasma clot culture even in the presence of Epo. Addition of citrated plasma to methylcellulose cultures inhibited Epo‐independent CFU E growth, but not Epo‐dependent growth, by both a normal and patients' bone marrow cells. These results demonstrate that bone marrow cells from some patients with sideroblastic anemia can undergo Epo‐independent and Epo‐dependent CFU E growth in vitro, and that there is a marked difference in CFU E growth depending on the type of clonal culture method used. It is suggested that the culture microenvironment, plasma, and sensitivity to Epo may be contributing factors which allow specific clones of sideroblastic anemia CFU E ‐forming cells to proliferate in vitro.