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Transferrin receptors on human reticulocytes: Variation in site number in hematologic disorders
Author(s) -
Shumak Kenneth H.,
Rachkewich Rose A.
Publication year - 1984
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830160104
Subject(s) - reticulocyte , transferrin , transferrin receptor , receptor , monoclonal antibody , soluble transferrin receptor , biology , medicine , endocrinology , immunology , antibody , biochemistry , anemia , iron status , iron deficiency , messenger rna , gene
Assays of binding of 125 iodine‐labeled ( 125 I) human transferrin were used to study transferrin receptor sites on reticulocytes from 15 normal subjects and from 66 patients with various hematologic disorders. In normal subjects, few or no transferrin receptors were detected whereas the average number of receptors per reticulocyte varied greatly from patient to patient, ranging from 0 to 67,700 in samples, from 35 patients, on which Scatchard analysis of binding of [ 125 I]‐transferrin was done. Marked heterogeneity in the number of reticulocyte transferrin receptors in different hematologic disorders was also found in assays with [ 125 I]‐OKT9 (monoclonal antibody to the human transferrin receptor). The number of receptors was not correlated with either the reticulocyte count or the hemoglobin.