Premium
A rapid in vitro drug‐sensitivity assay in acute nonlymphocytic leukemia
Author(s) -
Theodorakis Michael E.,
Goldberg Jack
Publication year - 1983
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830150307
Subject(s) - in vivo , in vitro , bone marrow , leukemia , drug , pharmacology , medicine , acute myelomonocytic leukemia , myeloid leukemia , myeloid , immunology , chemistry , biology , biochemistry , microbiology and biotechnology
Leukemic myeloid, myelomonocytic, and monocytic cells will incorporate radiolabeled sulfate into newly synthesized macromolecules. We developed a liquid culture technique to examine the in vitro effects of chemotherapeutic agents on the incorporation of radiolabeled sulfate into cells of patients with acute nonlymphocytic leukemia (ANLL). Cells recovered from bone marrow or peripheral blood of 25 patients with ANLL were incubated in vitro for one hour with saline (control) or a variety of chemotherapeutic agents. Cells were washed free of the drug and grown in liquid cultures containing nutrient medium and 35 SO 4 . The percent of 35 SO 4 incorporated into the drug treated as compared with control cells was determined after one, three, and seven days of culture. Patients whose drug‐treated cells incorporated less than 30% of 35 SO 4 when compared with the control after three or seven days of culture achieved a complete response to these agents in vivo (P < .05). Thus, the in vitro effects of various chemotherapeutic agents on the incorporation of 35 SO 4 into cells obtained from patients with ANLL may help predict clinical response to these agents in vivo.