Premium
Simultaneous detection of two mechanisms of immune destruction of penicillin‐treated human red blood cells
Author(s) -
Yust Israel,
Frisch Bertha,
Goldsher Naomi
Publication year - 1982
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830130107
Subject(s) - antibody dependent cell mediated cytotoxicity , phagocytosis , antiserum , peripheral blood mononuclear cell , immune system , cytotoxicity , penicillin , population , hemolysis , red blood cell , antibody , microbiology and biotechnology , chemistry , biology , opsonin , immunology , in vitro , biochemistry , medicine , antibiotics , environmental health
Two separate processes of putative red‐cell destruction in penicillin‐induced immune hemolysis were measured simultaneously by a rapid (3 hour) assay utilizing 51 Cr‐labelled red blood cells (RBC). Antibody‐dependent, cell‐mediated cytotoxicity (ADCC) was estimated by release of 51 Cr; and antibody‐dependent phagocytosis (ADPh) by quantitation of 51 Cr uptake into mononuclear phagocytes as well as by counts of engulfed RBC. Attacking cells were obtained by Ficoll‐Hypaque separation of peripheral blood from normal donors. Phagocytosis as well as lysis were proportional to anti‐penicillin antiserum concentration, to incubation time, and to the concentration of the attacking cells. Enrichment of mononuclear phagocytes in the attacking cell population by albumin gradient separation led to an increase in phagocytosis as well as in cytotoxicity. Depletion of mononuclear phagocytes resulted in a decline in both processes. Dilution of antiserum abolished ADCC but affected ADPh only slightly. Iodoacetate as well as colchicine inhibited both activities. These results indicate that both processes may be operative in the immune destruction of RBC in vivo.