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Simultaneous evaluation for terminal deoxynucleotidyl transferase and myeloperoxidase in leukemia
Author(s) -
Folds J. D.,
Bollum F. J.,
Dean L.,
Chan J.,
Stass S. A.
Publication year - 1982
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830120410
Subject(s) - terminal deoxynucleotidyl transferase , myeloperoxidase , chronic myelogenous leukemia , leukemia , chronic myelomonocytic leukemia , medicine , tunel assay , cancer research , immunology , immunohistochemistry , bone marrow , myelodysplastic syndromes , inflammation
A technique for dual staining of cells using terminal deoxynucleotidyl transferase (TdT) and myeloperoxidase (MPO) is described. The technique has been applied to cells of two patients. One patient had chronic myelomonocytic leukemia evolving into acute myelomonocytic leukemia. The other patient had chronic myelogenous leukemia in blast crisis. Our findings indicate that TdT and MPO are exclusive markers except for a rare precursor cell with dual staining in one patient. This study supports the concept of acute mixed leukemia.