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Monocyte α‐naphtyl esterase deficiency in chronic granulomatous disease
Author(s) -
GougerotPocidalo MarieAnne,
Buriot Diego,
Griscelli Claude,
Hakim Jacques
Publication year - 1981
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830100208
Subject(s) - nitroblue tetrazolium , zymosan , butyrate , monocyte , esterase , chronic granulomatous disease , stimulation , immunology , chemistry , medicine , enzyme , biochemistry , in vitro , fermentation
Monocytes from five unrelated children (four boys and a girl) with chronic granulomatous disease were studied for their ability to reduce nitroblue tetrazolium dye after stimulation with zymosan, and for their α‐naphtyl butyrate esterase activity. As expected, monocytes ingested zymosan particles but failed to reduce nitroblue tetrazolium dye. However, monocytes from two boys out of the five patients were α‐naphtyl butyrate esterase‐negative, whereas both their neutrophils and monocytes were positive for granular naphtol AS‐D esterase activity.