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Immunoassay of circulating terminal transferase in patients with acute lymphoblastic leukemia: A new technique for diagnosis
Author(s) -
Stass Sanford A.,
Peiper Stephen C.,
Bollum F.J.
Publication year - 1980
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830090410
Subject(s) - terminal deoxynucleotidyl transferase , immunoassay , medicine , lymphoblastic leukemia , bone marrow , leukemia , microbiology and biotechnology , antigen , immunology , antibody , pathology , tunel assay , biology , immunohistochemistry
Abstract The measurement of circulating terminal deoxynucleotidyl transferase (TdT) antigen can be accomplished by the use of an ELISA immunoassay system. TdT was present in either peripheral or bone marrow plasma of five patients with acute lymphoblastic leukemia (ALL) and absent from normal controls using ELISA. The assay was sensitive to a level of 20 ng/ml. The ALL patient values ranged from 160 ng/ml to 420 ng/ml. Use of ELISA for quantitation of TdT may have important diagnostic implications in patients with ALL.