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Prolymphocytic leukemia: Flow microfluorometric, immunologic, and cytogenetic observations
Author(s) -
Diamond Lawrence W.,
Bearman Robert M.,
Berry Paula K.,
Mills Bonnie J.,
Nathwani Bharat N.,
Weisenburger Dennis D.,
Winberg Carl D.,
Teplitz Raymond L.,
Rappaport Henry
Publication year - 1980
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830090311
Subject(s) - prolymphocytic leukemia , pathology , flow cytometry , staining , cytogenetics , leukemia , biology , dna , immunoglobulin d , microbiology and biotechnology , chronic lymphocytic leukemia , chromosome , immunology , b cell , medicine , antibody , genetics , gene
Cells isolated from four patients with prolymphocytic leukemia were evaluated by surface markers, cytogenetics, and flow microfluorometric analysis of cell size and DNA content. All four patients had B‐cell markers with a high density of IgM, kappa type, and Ia‐like antigen. Less intense staining for surface IgD was also observed. In each patient studied, chromosomal modes were in the hypodiploid or near‐diploid range. Despite the karyotypic abnormalities, the cellular DNA content, as determined by flow microfluorometry, was within normal limits in all cases. This suggests that the variability in chromosome numbers seen in these patients may reflect an abnormality in DNA packaging rather than differences in total DNA content. The modal electronic cell size of the prolymphocytes, determined by light scatter, was readily distinguishable from that of normal peripheral blood lymphocytes and the lymphocytes of chronic lymphocytic leukemia. Fewer than 4% of the peripheral prolymphocytes had S‐phase DNA content, a finding consistent with the chronic nature of this leukemia.