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A new density gradient system for the separation of human red blood cells
Author(s) -
Vettore Luciano,
De Matteis Maria Concetta,
Zampini Patrizia
Publication year - 1980
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830080307
Subject(s) - density gradient , chromatography , centrifugation , chemistry , polyvinylpyrrolidone , labelling , red blood cell , in vivo , aqueous solution , colloid , hemoglobin , biochemistry , biophysics , biology , polymer chemistry , physics , microbiology and biotechnology , quantum mechanics
Abstract A new density system for the separation of human red blood cells by density‐gradient centrifugation is described. The gradient medium is made with colloidal silica particles coated with polyvinylpyrrolidone suspended in aqueous solution of meglamine diatrizoate. By this method, more than 10 red‐cell fractions can be separated. These show different ages (by creatine content and 59 Fe in vivo labelling) and different characteristics (ie, potassium content). A 40‐fold enrichment in reticulocytes can be obtained in the top layers, with a great improvement of specific activity in labelling procedures of newly synthesized globin chains. The method is simple, rapid, inexpensive, reliable, nontoxic for erythrocytes, and is suitable for globin synthesis and other studies of erythrocyte metabolism.