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Characterization of platelets from normal mink and mink with the Chediak‐Higashi syndrome
Author(s) -
Meyers K. M.,
Holmsen H.,
Seachord C. L.,
Hopkins G.,
Gorham J.
Publication year - 1979
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830070206
Subject(s) - platelet , mink , chédiak–higashi syndrome , adenosine diphosphate , adenine nucleotide , adenosine triphosphate , nucleotide , extracellular , endocrinology , adenosine , biology , thrombin , biochemistry , medicine , chemistry , platelet aggregation , immunology , ecology , gene
Bleeding times of mink with the Chediak‐Higashi (CH) syndrome was markedly prolonged. Platelet counts were normal but there was an impaired platelet aggregation response to collagen. The metabolic adenine nucleotide pool of platelets from normal and CH mink was labeled with 14 C‐adenine and the platelets were gel‐filtered. Gel‐filtered platelets (GFP) from CH mink contained only 37.9% of the adenosine triphosphate (ATP) and 9.6% of the adenosine diphosphate (ADP) found in normal platelets and the ATP/ADP ratio was similar to the 14 C‐ATP/ 14 C‐ADP ratio. Platelet content of Ca 2+ , Mg 2+ , and in particular 5‐hydroxytryptamine was decreased. When GFP were incubated with thrombin to induce maximal secretion, only negligible amounts of ATP and ADP were released. The specific activity of the extracellular nucleotides approximated that within the platelet. These findings suggest that the stored nucleotide pool in CH platelets is virtually absent and that the abnormalities in platelet function may be due, in part, to the essential absence of secretable ADP and serotonin. The release of Ca 2+ and Mg 2+ by CH platelets was 56% and 27.8% of normal, respectively.