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Acid phosphatase in leukemic blasts: Characterization by isoelectric focusing in polyacrylamide gel
Author(s) -
Kass Lawrence,
Munster Donald
Publication year - 1979
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830060410
Subject(s) - isoelectric focusing , acid phosphatase , cytochemistry , isozyme , polyacrylamide gel electrophoresis , phosphatase , polyacrylamide , biochemistry , biology , microbiology and biotechnology , isoelectric point , chromatography , chemistry , enzyme
Using the high resolution technique of isoelectric focusing in polyacrylamide gel, isoenzymatic components of acid phosphatase were detected in cell‐free extracts prepared from different cytologic types of leukemic blasts in adults. Results indicate that for different cytologic types, different characteristic patterns of acid phosphatase isoenzyme could be detected. These studies extend conventional cytochemistry and indicate that characteristic patterns of acid phosphatase isoenzyme can be detected for various cytologic types of acute leukemia.