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Quantitation of globin chains using cellulose acetate electrophoresis: Analysis of fetal globin chain production
Author(s) -
Barton Jennifer,
Smith Merran B.,
Cauchi M. N.
Publication year - 1978
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830050409
Subject(s) - globin , cellulose acetate , chemistry , fetus , electrophoresis , chain (unit) , microbiology and biotechnology , biochemistry , chromatography , biology , pregnancy , cellulose , hemoglobin , genetics , physics , astronomy
Abstract A technique involving the quantitative estimation of globin chains using cellulose acetate electrophoresis is described, and applied to the analysis of fetal globin chain production. When cord blood was analyzed, there was poor correlation between the globin chain ratios namely β/α, γ/α and β/γ ratios with either age or weight of newborn babies between 36–40 weeks gestation: best correlations were obtained for the β/γ ratio against weight (P < 0.05). However, there was highly significant correlation between the various globin chain ratios and either age or weight of the newborn infant up to 12 weeks of age (P < 0.001). This technique allows visual demonstration and quantitation of abnormal haemoglobins in a single procedure.