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Permanent preparations of granulocyte macrophage cultures in agar gel
Author(s) -
Mendelow B.,
Grobicki D.,
De La Hunt M.,
Katz J.
Publication year - 1978
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830050211
Subject(s) - agar , incubation , staining , agar plate , agar gel , biology , differential staining , microbiology and biotechnology , biochemistry , bacteria , genetics
A simple method for the permanent preservation of agar cultures is described. The technique involves fixation of agar cultures after incubation, drying, and subsequent remounting and staining on glass slides. The technique permits differential counting of colony type, approximate quantitation of cell numbers within individual colonies or clusters, and permanent preservation of cultures for purposes of interlaboratory standardization, and for routine reference in patient records.