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Hemopoietic support capacity of the adult mouse liver: II. Studies in acetylphenylhydrazine‐treated mice
Author(s) -
Adler Solomon S.,
Trobaugh Frank E.
Publication year - 1978
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830040404
Subject(s) - haematopoiesis , spleen , biology , liver tissue , stem cell , immunology , medicine , andrology , endocrinology , microbiology and biotechnology
We investigated the hemopoietic support capacity of the liver in intact and splenectomized adult mice treated with three daily injections of acetylphenylhydrazine (APH). Packed red cell volumes, liver and spleen weights, numbers of pluripotent hemopoietic stem cells (CFU‐S) in blood and liver, and liver histology were evaluated 4, 8, 12, 16, and 20 days after the first injection. We found that 1) splenectomized, APH‐treated mice had a greater and more sustained increase in the weights of their livers than the increase found in livers of intact APH‐treated mice; 2) APH treatment elicited a much greater increase in the blood and liver CFU‐S of splenectomized mice (47 and 42 times normal, respectively) than it elicited in the blood and liver CFU‐S of intact mice (4–5 and 4 times normal, respectively); and 3) APH treatment induced numerous foci of hemopoietic tissue in the livers of splenectomized mice. The results of the CFU‐S studies can be explained by, and to some extent support, the thesis that the adult mouse liver does not support proliferation of normal CFU‐S, but can trap large numbers of circulating CFU‐S. In addition, these studies suggest that the livers of adult mice are able to support only limited proliferation of differentiated hemopoietic elements.