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In vitro functional capabilities of canine polymorphonuclear neutrophils collected simultaneously by continuous‐flow centrifugation and continuous‐flow filtration leukopheresis
Author(s) -
Cook Lloyd O.,
Boxer Laurence A.,
Allen John M.,
Griep John A.,
Baehner Robert L.
Publication year - 1978
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830040304
Subject(s) - centrifugation , degranulation , leukapheresis , chemotaxis , in vitro , filtration (mathematics) , biology , granulocyte , microbiology and biotechnology , chemistry , immunology , biochemistry , statistics , mathematics , receptor , stem cell , cd34
Polymorphonuclear neutrophils were simultaneously collected from dogs by continuous‐flow centrifugation and continuous‐flow filtration leukapheresis. In vitro studies were performed on cells obtained by the two methods as well as on control cells. Studies consisted of assessment of phagocytic capacity, degranulation, chemotaxis, hexose monophosphate (HMP) shunt activity, and bacterial killing. The cells obtained from the filter were metabolically more active than those harvested by centrifugation, as evidenced by increase in resting HMP shunt activity and dimunition in total available lysozyme‐secreting activity compared to centrifuged cells. Despite their impaired phagocytic capacities, the filtered cells were able to kill Staphylococcus aureus as efficiently as the centrifuged cells. Both cell populations responded to chemotactic gradients equally.