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Non‐leukemic dividing cells in the blood of leukemic patients
Author(s) -
Humbert James R.,
Morse Helvise G.,
Hutter John J.,
Rose Barbara,
Robinson Arthur
Publication year - 1978
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830040303
Subject(s) - mitosis , leukemia , karyotype , bone marrow , biology , acute lymphocytic leukemia , immunology , pathology , medicine , lymphoblastic leukemia , genetics , chromosome , gene
Spontaneous mitoses in the blood of 67 patients with acute leukemia were enumerated and their identity determined by cytogenetic methods. Most patients were children with acute lymphoblastic leukemia. Simultaneous 16‐to 20‐hour cultures of blood leukocytes (B u ) and of bone marrow (BM) cells were performed without phytohemagglutinin (PHA). Blood leukocytes were also cultured with PHA for 72 hours (B PHA ). Mitoses in B u cultures were counted, and karyotypic analysis performed on cells from the three culture types. In 21 control subjects, B u cultures usually yielded no mitoses. Relapse and remission patients both displayed significantly more B u mitoses than the controls. The karyotypes of B u , B PHA , and BM mitoses in remission patients were normal. Fifty percent of relapse patients displayed cytogenetically abnormal leukemia cell lines; the percentage of their abnormal karyotypes was significantly higher in BM cells than in B u or B PHA cells. The majority of the mitotic cells in B u cultures from both relapse and remission patients appear to be of a nonleukemic origin. The number of mitoses could not be correlated with type of leukemia, hematologic parameters, or prognosis.