Premium
In vivo and in vitro effects of thrombin and plasmin on human factor VIII (ahf)
Author(s) -
Lian Eric ChunYet,
Nunez Roger L.,
Harkness Donald R.
Publication year - 1976
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830010413
Subject(s) - medicine , heparin , coagulation , thrombin , plasmin , pulmonary embolism , myocardial infarction , antigen , in vivo , disseminated intravascular coagulation , immunoelectrophoresis , gastroenterology , immunology , platelet , chemistry , biochemistry , enzyme , biology , microbiology and biotechnology
The relationship between factor VIII (AHF) procoagulant activity and factor VIII‐related antigen were examined in patients with disseminated intravascular coagulation (DIC), pulmonary embolism (PE), and coronary artery disease with or without myocardial infarction (MI). It was found that 13 of 13 patients with DIC, 17 of 17 patients with PE, and 10 of 12 patients with MI possessed a significantly elevated factor VIII‐related antigen to factor VIII activity ratio (VIII‐ratio). The VIII‐ratio returned to normal in each of 2 patients with DIC and 1 patient with PE after treatment with heparin, heparin and α‐amino‐caproic acid, and heparin and coumadin respectively. In contrast, the VIII‐ratio was slightly elevated only in 1 of 15 patients with coronary artery insufficiency without MI. In in vitro studies, after treatment of plasma with thrombin or plasmin, factor VIII activity was lost, whereas the amount of factor VIII‐related antigen remained the same or was even increased when measured by agarose quantitative immunoelectrophoresis. These observations have led us to conclude that an elevated VIII‐ratio is a very sensitive indicator of intravascular coagulation.