Premium
Bone marrow hematons: An access point to the human hematopoietic niche
Author(s) -
Janel Alexandre,
Berger Juliette,
Bourgne Céline,
Lemal Richard,
BoiretDupré Nathalie,
DuboisGalopin Frédérique,
Déchelotte Pierre,
Bothorel Charlotte,
Hermet Eric,
Chabi Sara,
Bay JacquesOlivier,
Lambert Céline,
Pereira Bruno,
Pflumio Françoise,
Haddad Rima,
Berger Marc G.
Publication year - 2017
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.24830
Subject(s) - haematopoiesis , cd34 , bone marrow , stem cell , myeloid , progenitor cell , biology , hematopoietic stem cell , immunology , mesenchymal stem cell , niche , cancer research , microbiology and biotechnology , ecology
To understand the complex interactions between hematopoietic stem cells and the bone marrow niche, a human experimental model is needed. Our hypothesis is that hematons are an appropriate ex vivo model of human bone marrow. We analyzed the hierarchical hematopoietic cell content and the tissue organization of single hematons from healthy donors. Most (>90%) hematons contained precursors of all cell lineages, myeloid progenitors, and LTC‐ICs without preferential commitment. Approximately, half of the hematons could generate significant levels of lympho‐myeloid hematopoiesis after transplantation in an NSG mouse model, despite the low absolute numbers of transplanted CD34 + cells. Mesenchymal STRO‐1 + and/or CD271 + cells formed a critical network that preserved hematon cohesion, and STRO‐1 + cells colocalized with most hematopoietic CD34 + cells (68%). We observed an influence of age and gender. These structures represent a particularly attractive model for studying the homeostasis of the bone marrow niche and pathological changes that occur during diseases.