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A flow cytometry‐based assay for measuring invasion of red blood cells by Plasmodium falciparum
Author(s) -
Bei Amy K.,
DeSimone Tiffany M.,
Badiane Aida S.,
Ahouidi Ambroise D.,
Dieye Tandakha,
Ndiaye Daouda,
Sarr Ousmane,
Ndir Omar,
Mboup Souleymane,
Duraisingh Manoj T.
Publication year - 2010
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.21642
Subject(s) - parasitemia , parasite hosting , flow cytometry , plasmodium falciparum , biology , malaria , cytometry , red blood cell , staining , in vitro , immunology , virology , microbiology and biotechnology , genetics , world wide web , computer science
Variability in the ability of the malaria parasite Plasmodium falciparum to invade human erythrocytes is postulated to be an important determinant of disease severity. Both the parasite multiplication rate and erythrocyte selectivity are important parameters that underlie such variable invasion. We have established a flow cytometry‐based method for simultaneously calculating both the parasitemia and the number of multiply‐infected erythrocytes. Staining with the DNA‐specific dye SYBR Green I allows quantitation of parasite invasion at the ring stage of parasite development. We discuss in vitro and in vivo applications and limitations of this method in relation to the study of parasite invasion. Am. J. Hematol., 2010. © 2010 Wiley‐Liss, Inc.