Premium
Purification of CD45+ hematopoietic cells directly from human bone marrow using a flow‐based P‐selectin‐coated microtube
Author(s) -
Narasipura Srinivas D.,
Wojciechowski Joel C.,
Duffy Brian M.,
L. Liesveld Jane,
King Michael R.
Publication year - 2008
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.21204
Subject(s) - bone marrow , cd34 , haematopoiesis , progenitor cell , stem cell , flow cytometry , human bone , cell , hematopoietic stem cell , immunology , microbiology and biotechnology , cancer research , chemistry , medicine , biology , in vitro , biochemistry
Recently, a flow‐based selectin‐dependent method for capture and enrichment of specific type blood cells (CD34+ hematopoietic stem and progenitor cells) from bone marrow and peripheral blood has been described. Using a similar approach, here we show isolation of CD45+ blood cells directly from human bone marrow to a high purity (90%–97%). The process mimics a ubiquitous mechanism of cell trafficking in the body for the recruitment of neutrophils during inflammation. The method is straightforward, rapid, and may represent a practical alternative to CD45+ cell enrichment procedures required for chimerism analysis following allogenic stem‐cell transplantation. Am. J. Hematol., 2008. © 2008 Wiley‐Liss, Inc.