Purification of CD45+ hematopoietic cells directly from human bone marrow using a flow‐based P‐selectin‐coated microtube | Zendy

Narasipura Srinivas D. | Zendy; Wojciechowski Joel C. | Zendy; Duffy Brian M. | Zendy; L. Liesveld Jane | Zendy; King Michael R. | Zendy

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Purification of CD45+ hematopoietic cells directly from human bone marrow using a flow‐based P‐selectin‐coated microtube

Author(s) -

Narasipura Srinivas D.,

Wojciechowski Joel C.,

Duffy Brian M.,

L. Liesveld Jane,

King Michael R.

Publication year - 2008

Publication title -

american journal of hematology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.456

H-Index - 105

eISSN - 1096-8652

pISSN - 0361-8609

DOI - 10.1002/ajh.21204

Subject(s) - bone marrow , cd34 , haematopoiesis , progenitor cell , stem cell , flow cytometry , human bone , cell , hematopoietic stem cell , immunology , microbiology and biotechnology , cancer research , chemistry , medicine , biology , in vitro , biochemistry

Recently, a flow‐based selectin‐dependent method for capture and enrichment of specific type blood cells (CD34+ hematopoietic stem and progenitor cells) from bone marrow and peripheral blood has been described. Using a similar approach, here we show isolation of CD45+ blood cells directly from human bone marrow to a high purity (90%–97%). The process mimics a ubiquitous mechanism of cell trafficking in the body for the recruitment of neutrophils during inflammation. The method is straightforward, rapid, and may represent a practical alternative to CD45+ cell enrichment procedures required for chimerism analysis following allogenic stem‐cell transplantation. Am. J. Hematol., 2008. © 2008 Wiley‐Liss, Inc.

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