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Coincidental outbreak of methicillin‐resistant Staphylococcus aureus in a hematopoietic stem cell transplantation unit
Author(s) -
Imataki Osamu,
Makimoto Atsushi,
Kato Shingo,
Bannai Takahiro,
Numa Naomi,
Nukui Yoko,
Morisawa Yuji,
Ishida Toshihiko,
Kami Masahiro,
Fukuda Takahiro,
Mori Shinichiro,
Tanosaki Ryuji,
Takaue Yoichi
Publication year - 2006
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.20668
Subject(s) - outbreak , pulsed field gel electrophoresis , hematopoietic stem cell transplantation , staphylococcus aureus , methicillin resistant staphylococcus aureus , typing , transplantation , medicine , staphylococcal infections , amplified fragment length polymorphism , microbiology and biotechnology , virology , biology , genotype , genetics , bacteria , population , genetic diversity , environmental health , gene
Background: Methicillin‐resistant Staphylococcus aureus (MRSA) is one of the most common nosocomial pathogens among hospital‐acquired infections, and immunocompromised patients are highly susceptive to infection. The molecular typing of isolated strains is a common method for tracing an outbreak of MRSA, but experience with this approach is still limited in the hematopoietic stem cell transplantation (HSCT) ward. Methods: We experienced 6 cases of MRSA infection/colonization in our 26‐bed HSCT ward during a 4‐week period. This unusual outbreak strongly suggested that the same MRSA strain was involved despite strict isolation and aseptic patient care. Clarification of the transmission pattern was critical, and we applied pulsed‐field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP) assays for evaluation. Results and conclusion: In four of the six cases, the pattern of bands examined by PFGE and AFLP analyses supported the idea that direct person‐to‐person transmission was very unlikely and the outbreak was coincidental. This experience highlights the clinical value of molecular typing methods for the clinical epidemiological assessment of MRSA outbreak. Am. J. Hematol., 2006. © 2006 Wiley‐Liss, Inc.