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Clonal T cells of pure red‐cell aplasia
Author(s) -
Masuda Michihiko,
Teramura Masanao,
Matsuda Akira,
Bessho Masami,
Shimamoto Takashi,
Ohyashiki Kazuma,
Omine Mitsuhiro,
Motoji Toshiko,
Mizoguchi Hideaki
Publication year - 2005
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.20374
Subject(s) - pure red cell aplasia , t cell receptor , polymerase chain reaction , blot , microbiology and biotechnology , aplasia , medicine , kappa , gastroenterology , biology , immunology , t cell , pathology , genetics , gene , anemia , linguistics , philosophy , immune system
This study detected clonal T cells in patients with acquired pure red‐cell aplasia (PRCA) by Southern blotting and polymerase chain reaction (PCR). Twenty‐nine adult patients with acquired PRCA were enrolled in this study. Seventeen patients had primary acquired PRCA, while 12 patients had the secondary form. Twenty‐two of 29 (76%) patients demonstrated TCR rearrangement by at least one method. We divided the patients into three groups depending on T‐cell clonality. The CD4/8 ratio of patients who were positive on Southern blotting was significantly lower than that of other groups. Except for the CD4/8 ratio, other laboratory findings did not significantly differ among the three groups. The CD4/8 ratio should be a useful surrogate marker to detect T‐cell clonality. Am. J. Hematol. 79:332–333, 2005. © 2005 Wiley‐Liss, Inc.