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Relationship between Thy‐1 expression and cell‐cycle distribution in human bone marrow hematopoietic progenitors
Author(s) -
Takeda Hiroki,
Yamamoto Masuji,
Morita Naoko,
Tanizawa Takakuni
Publication year - 2005
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.20362
Subject(s) - haematopoiesis , bone marrow , progenitor cell , human bone , hematopoietic cell , distribution (mathematics) , cell cycle , expression (computer science) , biology , immunology , medicine , cell , microbiology and biotechnology , stem cell , genetics , computer science , mathematics , mathematical analysis , in vitro , programming language
Analysis of the relationship between Thy‐1 expression and cell‐cycle distribution of hematopoietic stem cells (HSCs) showed that freshly isolated Thy‐1 + and Thy‐1 − subsets of the CD34 high CD38 − flt‐3 − Lin − population were predominantly in G 0 /G 1 phase and remained essentially quiescent, whereas after 6 days of cytokine stimulation, the Thy‐1 + subset of the population entered the cycling state while the Thy‐1 − subset again remained quiescent. Expression of Thy‐1 antigen resulted in a drastic increase in the percentage of cycling cells in CD34 high CD38 ‐ flt‐3 ‐ Lin ‐ Thy‐1 + ‐ as well as CD34 high CD38 − flt‐3 − Lin − Thy‐1 − ‐cell‐initiated cultures. The Thy‐1 + subset of the CD34 high CD38 − flt‐3 − Lin − population exists in the freshly isolated CD34 high CD38 − flt‐3 − Lin − Thy‐1 + fraction, loses Thy‐1 expression during 6 days, and re‐expresses Thy‐1 for an additional 2 days. Cell‐cycle analysis demonstrated that this unique subset contains abundant S/G 2 M cells. Thus, Thy‐1 expression appears to be an indicator of cell‐cycle phase in targeting HSC, which might serve in the cell subset best suited for gene transfer. Am. J. Hematol. 79:187–193, 2005. © 2005 Wiley‐Liss, Inc.